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Image Search Results
Journal: Journal of Gastrointestinal Oncology
Article Title: The roles and mechanisms of APOL1 in the development of colorectal cancer
doi: 10.21037/jgo-24-275
Figure Lengend Snippet: Effect of APOL1 on the biological behavior of CRC cells. (A) qRT-PCR was used to determine the APOL1 mRNA levels in HCT116, SW1116, and NCM460 cells. (B) In HCT116 cells, qRT-PCR was used to determine the interference efficacy of shAPOL1#1/2/3. (C) CCK8 assay was used to evaluate the proliferative capacity of HCT116 and SW1116 cells. (D) The count of clones in HCT116 and SW1116 cells was determined using the colony formation test. Staining method: crystal violet. Magnification: ×100. (E) Wound healing test was used to assess the migratory capacity of HCT116 and SW1116 cells (magnification: ×40). (F) The Transwell test was used to determine HCT116 and SW1116 cells’ invasion ability (magnification: ×100). Staining method: crystal violet. Each group, n=3. *, P<0.05 vs. NCM460 group; **, P<0.01; ***, P<0.001 vs . sh-NC group. sh-NC, shRNA-negative control; sh-APOL1, shRNA-apolipoprotein L1; OD, optical density; CRC, colorectal cancer; qRT-PCR, quantitative real-time polymerase chain reaction; CCK8, Cell Counting Kit 8.
Article Snippet: Shanghai iCell Bioscience Inc. (Shanghai, China) provided the human
Techniques: Quantitative RT-PCR, CCK-8 Assay, Clone Assay, Staining, shRNA, Negative Control, Real-time Polymerase Chain Reaction, Cell Counting